An automated method for serum bilirubin determination
نویسندگان
چکیده
منابع مشابه
An automated method for serum bilirubin determination.
A method for the determination of both direct and indirect serum bilirubin on the AutoAnalyzer is described which has a number of advantages over the automated method that is currently recommended. It gives total bilirubin results which are similar to those obtained with the equivalent manual method and direct bilirubin results which are usually about 10% lower.The effect of haemolysis on estim...
متن کاملAn Automated Method for the Determination of Serum Bilirubin.
The bilirubin method of Rand and DiPasqua (3), which utilizes a stabilized diazonium salt solution of 2,4 dichloraniline, has been adapted to automatic chemical analysis. Both total bilirubin and “1-mm. reacting” bilirubin can be assayed with good accuracy and precision. Data are presented to show excellent agreement with the manually performed procedure and the procedure of Malloy and Evelyn (...
متن کاملEffect of bilirubin and lipemia on an automated method for serum cholesterol.
A simple automated procedure for total serum cholesterol, employing a stable Liebermann-Burchard reagent, was tested. The method has good precision, and results agree well with extraction methods for total cholesterol on normal serums. Serum samples with elevated bilirubin levels gave cholesterol values which were too high and which could not always be corrected for. Lipemic serums in some inst...
متن کاملAn ultramicro colorimetric method for determination of total and direct serum bilirubin.
A simple reproducible method for the ultramicro determination of total and direct serum bilirubin is presented. This method provides a greater sensitivity than that obtained with other colorimetric ultramicro procedures. Total bilirubin values agree with those obtained with the macro Malloy-Evelyn procedure, but direct-reading bilirubin values were significantly higher with the presented method.
متن کاملAutomated method for determination of serum 5'-nucleotidase activity.
An automated method has been devised for determining 5’-nucleotidase activity in serum. A modified creatine phosphokinase cartridge (Technicon Instrument Corp., Tarrytown, N. Y.) is used to prepare a reaction mixture containing, per liter: barbital buffer (pH 7.5), 16.1 mmol; adenosine-5’-phosphate, 5.4 mmol; Mg2, 20.2 mmol; phenyl phosphate, 7.8 mmol; adenosine deamlnase, 0.78 mg; and sample, ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1968
ISSN: 0021-9746
DOI: 10.1136/jcp.21.2.196